Primary source: http://pubs.acs.org/stoken/presspac/presspac/full/10.1021/ef9015872
Secondary source: http://www.sciencedaily.com/releases/2010/06/100609111318.htm
The article that I have chosen deals with the growing concern and need for renewable and accessible fuels; in particular the article focuses on the extraction of biodiesel from fungus to be used in renewable fuels. There is a growing concern in the global community about the rapid depletion and increasing scarcity of petroleum, the life blood of the modern world. Alternative fuels have been brought forth that have been synthesised from oils found in soy, sunflower seeds and palm. Though these fuels are clean burning and efficient, known as biodiesel, they bring up controversies about the use of edible food for fuel. The article being analysed addresses a ground breaking alternatives to the places that we find these oils needed for biodiesel; within microorganisms such as fungi.
Both the primary and secondary sources address the depletion of fossil fuels and the alternatives of fungi extracted biodiesel. However, the secondary source gives only a brief overview of both the research being done and the process by which the biomass extraction is being transformed into useable biodiesel. The secondary source also only mentions one specific fungi species being used in the research, being the one given the most attention and study, when, in fact, there are several species of fungi and microorganisms being tested and processed for the extracting of lipids and biomass. The primary source addresses the variety of organisms being studied but pays particular attention to the species Mucor circinelloid. The primary source mentions that it is due the lack of need for arable land and sunlight as well as no food completion that make oleaginous microorganisms such as fungi ideal for mass commercial production and research. The research paper then goes on to explain why the use of this particular fungi is used for the extraction of lipids. It explains that this is one of the only fungi in the study that has its genome completely sequenced and this allows for more in depth analysis of the of the fungi’s ability to produce such useful lipids. Connected to this the secondary source fails to mention the fact that the genome of the M. circinelloid is altered by replacing a certain mutant allele leuA with a wild allele. This genetic modification is only mentioned briefly in the primary research paper; this may pose more difficulty to the mass production of biodiesels in the future if genetic modification is required.
How exactly do they extract the biodiesel from the fungus to be used for fuel? The secondary source article doesn’t even so much as mention the process by which the lipids are extracted, except for a brief mention that the biodiesels are extracted without even extracting oils. This process is brought into full explanation by the primary article, though there are several weaknesses within their description of this process. The article describes the process as being a single- step method that transforms the oils within the biomass of the microorganism into useable fatty acid methyl esters, or FAME’s for short. This process of oil transformation is a little more complicated than the article seems to suggest at first. Once the reader gets past the complexity of the scientific terms, language and equations being used they can see that the process of FAME extraction involves incubation in a cocktail mixture of chemicals. This incubation also involves being kept in the dark for up to 96 hours and being spun at a constant of 250 spm. The solution then requires monitoring every 24 hours in order to keep the ph level steady with NaOH. The process continues through an acid-catalyzed transesterifictaion involving catalyst such as Br3, H2SO4 and HCl. A reagent – solvent system mixture of methanol and chloroform was added and placed in a rotary evaporator. The using various gases the residue of FAME’s was extracted and we are finally left with our desired produced to be used in the creation of biodiesel. As you can see this is much more than a one step process and more complicated than both the secondary source touches on and the primary sources pre determines. The matter of time also become a factor as both sources fail to mention the time requirement need for just one preparation of FAME’s ; this near week long process can pose a threat to the commercial production of biodiesel unless more efficient ways of extraction are developed.
The whole process involves the oils found in the fungus, but the secondary source does not specify which oils are involved. There are many different oils found in M. Circinelloides but only a small group of free fatty acids are suitable for processing in the acid-catalyzed transesterification. The oils percentages of the free fatty acids are also pretty abundant, but not quite as abundant as other oils and lipids found in the fungus.
Both the primary and secondary sources provide useful information on the research being done in the development of biodiesels from micro organic biomass. Though both these sources have useful strengths supporting the research they contain weaknesses in their description of the process of extraction and, as seen in the secondary source, in the lack of detail divulged. And BOOM goes the dynamite!!